@dc42 Okay, thanks for the help and fast replies!

In your config.g file, try changing your M106 commands to M106 P0 S0 I0 F500 H-1; and M106 P1 S0 I0 F500 H-1;

https://reprap.org/wiki/G-code

That wiki is a great source for learning about how to properly configure your printer

I do have a jumper across the INT_5V_EN pins. I've left the board sit for a couple hours, and now when plugged into via USB the 5V, Diag, and 3.3V lights flash briefly and immediately fade out.
When powered by a 12V source across VIN, the blue VIN and red 5V leds light up, but 3.3V is still dead.

I recently received a Duet Wifi V1.04, this is the second Duet board I've worked with. My wifi module initially was not powering on, I noticed a botch wire that was leading from the ground pin of the Wifi module to ground on the SD card reader. The botch wire was not properly soldered on the wifi side, easy fix, module started working.
I left the board sit over night-powered off-and wired the board to my 12V power supply this morning.
The green 3.3V led is not powering on when the board is powered by a 12V power supply, but does power on when the board is connected via usb.
No components on the board are getting hot, and nothing is connected to the board.
Any help with figuring out what is wrong is greatly appreciated!