I found out during printing and then researched on this and other forums. Doubble checked nearly everything which came out out.
When I push the DWC buttons or sending a gcode command it is the same behavior.
I already checked the wiring and everything looks fine to me.
I did not test with removed the Duex5, due to the fact that my bltouch is on the DUEX5.
What I can tell is that when I only use the Duet2 Wifi extruder ports, it is the same behaviour.