@jbjbjb82 said in Duet 2 Wifi Fans setup:

Can I connect the positive to the 5V pin of the Jumper Select and negative to the GND pin of the FAN1 connector? If so, will this allow to use thermostatic control, so that this 5V fan start running at 45ºC?

Yes. The Duet switches PWM on the negative side, so as long as the negative wire of the fan is connected to Fan1 you will be able to control it regardless of where it gets it's voltage source from.

When using multiple fan voltages in the same system be very careful not to short out a higher voltage into the 5v side.