Now i have solve the problem:
i have changed in Prusaslicer the Firmware G-Code Typ to "RepRap/Sprinter"
Then i add this customised Start G-code
;
; start script for PrusaSlicer for CaribouDuet
;
; =========================================================================================================
;
G0 X60 Y-3 Z80 ; move extruder above bed, keep extruder in front for cleaning and checking
;
M104 S160 T0 ; pre-heat extruder to 160°C
M140 S[first_layer_bed_temperature] ; this will take the layer 1 temperature for bed 0
M190 S[first_layer_bed_temperature] ; wait for bed temp
;
G29 ; mesh bed leveling using defined mesh grid
G0 X0 Y-3 Z0.6 ; go outside print area
;
M104 S[first_layer_temperature] ; set extruder temperature
M109 S[first_layer_temperature] ; wait for extruder temp
;
; =========================================================================================================
;
;
M98 P"0:/sys/primeLine.g" ; execute primeline macro
;
G92 E0.0 ; set extruder position
;
M572 D0 S0.07 ; set pressure advance
;
; =========================================================================================================
Now it works!