@Jords27 the binary files generated by Duet3Expansion projects are written to sit in flash memory above the bootloader, except for some of the debug variants and the no-bootloader variant. If you intend to program the firmware directly into the MCU without a bootloader then you will need to change the linker script file to target them to the bottom of flash memory instead. The linker scripts for SAME5x-based boards are in folder src/Hardware/SAME5x_C21/SAME5x.