@ameigh710 If your thermistor is reading 100k ohms at room temperature (about 25C) that's about right. You can't really read it when connected to the Duet, as you'll read the extra circuitry in the Duet, so a different reading is normal. You will get an error if you disconnect the thermistor while the Duet is on. With the thermistor disconnected, you should get a reading of -273C, and with the thermistor pins shorted (use a jumper or attach a wire, rather than poking a screwdriver in there) it will read 2000C.
Do you know what the thermistor make and model actually is? Depending on the thermistor, you may not need a C parameter.
If you're still getting an error, please post your config.g, and response to M115 to show firmware version and Duet hardware. Most likely it's a configuration error.
Ian